CBSE Class 12 Biotechnology Syllabus 2024-25 (PDF Download)

CBSE Class 12 Biotechnology Syllabus 2024-25


One Paper
Max. Marks 70+30
Time: 3 hrs. 




Unit V

Protein and Gene Manipulation


Unit VI

Cell Culture and Genetic Manipulation









One paper
Time: 3 hrs.
Total Marks: 70 

Unit-V Protein and Gene Manipulation                                  40 Marks

Chapter-1: Recombinant DNA Technology

Introduction, Tool of Recombinant DNA technology, Making rDNA molecule, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), DNA Sequencing.

Chapter-2: Protein Structure and Engineering

Introduction  to  the   world    of    proteins,   Structure-function          Relationship   in  proteins, Characterization of proteins, Protein based products, Designing proteins (Protein Engineering)

Chapter-3: Genomics, Proteomics and Bioinformatics

Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, Information sources, Analysis using bioinformatics tools.

Unit-VI Cell Culture and Genetic Manipulation                         30 Marks

Chapter-1: Microbial Cell Culture and its Applications

Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology.

Chapter -2: Plant Cell Culture and Applications

Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Transgenic plants with beneficial traits, Biosafety of transgenic plants

Chapter-3: Animal Cell Culture and Applications

Introduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell technology.


30 Marks

Note: Every student will be required to do the following experiments during the academic session.

  1. Use of special equipment in biotechnology experiments
  2. Isolation of bacterial plasmid DNA
  3. Detection of DNA by gel electrophoresis
  4. Estimation of DNA by UV spectroscopy
  5. Isolation of bacteria from curd & staining of bacteria
  6. Cell viability assay using Evan’s blue dye exclusion method
  7. Data retrieval and database search using internet site NCBI and download a DNA and protein sequence from internet, analyze it and comment on it
  8. Reading of a DNA sequencing gel to arrive at the sequence
  9. Project work

Scheme of Evaluation

Time: 3 Hours
Max. Marks 30

The scheme of evaluation at the end of the session will be as under:


Two experiments

6+6 (only one computer based practical)


Practical record



Viva on Practical



Project work


Write up



Viva on project





Note:- More emphasis should be given on hands on work in projects.

Prescribed Books:

  1. A Text Book of Biotechnology – Class XI : Published by CBSE, New Delhi
  2. As reference- Biotechnology – Class XI : Published by NCERT, New Delhi
  3. A Laboratory Manual of Biotechnology – Class XI : Published by CBSE, New Delhi
  4. A Text Book of Biotechnology – Class XII : Published by CBSE, New Delhi
  5. A Laboratory Manual of Biotechnology – Class XII : Published by CBSE, New Delhi

Assessment Areas (Theory) 2024-25 Class XII

Biotechnology (045)

Time : 3 hrs.
Maximum Marks: 70 Marks 



Demonstrate, Knowledge and Understanding


Application of Knowledge / Concepts


Analyse, Evaluate and Create



  • Typology of questions: VSA including MCQs, Assertion – Reasoning type questions; SA; LA-I; LA-II; Source-based/ Case-based/ Passage-based/ Integrated assessment questions.
  • An internal choice of approximately 33% would be
  • Suggestive verbs for various competencies
  • Demonstrate, Knowledge and Understanding State, name, list, identify, define, suggest, describe, outline, summarize, etc.
  • Application of Knowledge/Concepts Calculate, illustrate, show, adapt, explain, distinguish, etc.
  • Analyze, Evaluate and Create Interpret, analyse, compare, contrast, examine, evaluate, discuss, construct, etc.

Download PDF

Download Syllabus PDFClick here
Complete SyllabusClass 12 Syllabus